Multibatch Cytometry Data Integration for Optimal Immunophenotyping

Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium

Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, eight-color panels for identification of major immune cell subsets in peripheral blood. These were produced as pre-configured, lyophilized, reagents...

Diagnostic usefulness of flow cytometry for immunophenotyping classical Hodgkin lymphoma.

Am J Clin Pathol 2011;136:156-158 157 © American Society for Clinical Pathology high-fluorescence nucleated cells that may appear in CSF under certain conditions (eg, neoplasms or leptomeningeal metastases, macrophages, and eosinophils). These conclusions are similar to those drawn by other authors4 who have found most patients with low cell counts (<20/μL) to be correctly classified. Obviously...

Flow cytometry immunophenotyping for diagnosis of myelodysplastic syndrome.

A practical approach to multicolor flow cytometry for immunophenotyping.

Through a series of novel developments in flow cytometry hardware, software, and dye-chemistry it is now possible to simultaneously measure up to 11 distinct fluorescences and two scattered light parameters on each cell. Such advanced multicolor systems have a number of advantages over current two- and three-color flow cytometric measurements. They provide a large amount of novel information fo...

Normative data for flow cytometry immunophenotyping of benign lymph nodes sampled by surgical biopsy

AIMS To create clinically relevant normative flow cytometry data for understudied benign lymph nodes and characterise outliers. METHODS Clinical, histological and flow cytometry data were collected and distributions summarised for 380 benign lymph node excisional biopsies. Outliers for kappa:lambda light chain ratio, CD10:CD19 coexpression, CD5:CD19 coexpression, CD4:CD8 ratios and CD7 loss w...